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BRG1 helps RNA polymerase II to overcome a nucleosomal barrier during elongation, in vivo.

ABSTRACT: RNA polymerase II (RNAPII) transcribes genes in a chromatin context. We have designed a system to investigate the role of chromatin remodelling during elongation in vivo, which involves inserting a strong nucleosome-positioning sequence between a promoter and a reporter gene. Our data indicate that a nucleosome positioned in the body of a transcription unit impairs RNAPII progression, provokes RNAPII accumulation upstream to the positioned nucleosome and reduces transcription. By using this system, we show that BRG1, the enzymatic motor of the SWI-SNF chromatin-remodelling complex, is recruited to the positioned nucleosome in a transcription elongation-dependent manner and facilitates traversal of the nucleosome by RNAPII.

SUBMITTER: Subtil-Rodriguez A

PROVIDER: S-EPMC2948185 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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BRG1 helps RNA polymerase II to overcome a nucleosomal barrier during elongation, in vivo.

Subtil-Rodríguez Alicia A Reyes José C JC

EMBO reports 20100910 10

RNA polymerase II (RNAPII) transcribes genes in a chromatin context. We have designed a system to investigate the role of chromatin remodelling during elongation in vivo, which involves inserting a strong nucleosome-positioning sequence between a promoter and a reporter gene. Our data indicate that a nucleosome positioned in the body of a transcription unit impairs RNAPII progression, provokes RNAPII accumulation upstream to the positioned nucleosome and reduces transcription. By using this syst ...[more]

PMID: 20829883

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