Molecular detection and characterization of Brucella species in raw informally marketed milk from Uganda.
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ABSTRACT:
SUBMITTER: Hoffman T
PROVIDER: S-EPMC5107632 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
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Molecular detection and characterization of <i>Brucella</i> species in raw informally marketed milk from Uganda.
Hoffman Tove T Rock Kim K Mugizi Denis Rwabiita DR Muradrasoli Shaman S Lindahl-Rajala Elisabeth E Erume Joseph J Magnusson Ulf U Lundkvist Åke Å Boqvist Sofia S
Infection ecology & epidemiology 20161111
This study identified and characterized <i>Brucella</i> species in the informal milk chain in Uganda. A total of 324 cattle bulk milk samples were screened for the genus <i>Brucella</i> by real-time PCR with primers targeting the <i>bcsp31</i> gene and further characterized by the <i>omp25</i> gene. Of the samples tested, 6.5% were positive for <i>Brucella</i> species. In the <i>omp25</i> phylogeny, the study sequences were found to form a separate clade within the branch containing <i>B. abortu ...[more]
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