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Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging.

ABSTRACT: The complete genome sequence of an Australian isolate of Turnip mosaic virus was determined by Sanger sequencing. After seven years of serial passaging by mechanical inoculation, the isolate was resequenced by RNA sequencing (RNA-Seq). Eighteen single nucleotide polymorphisms were identified between the isolates. Both isolates had 96% identity to isolate AUST10.

SUBMITTER: Pretorius L

PROVIDER: S-EPMC5114374 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging.

Pretorius Lara L Moyle Richard L RL Dalton-Morgan Jessica J Hussein Nasser N Schenk Peer M PM

Genome announcements 20161117 6

The complete genome sequence of an Australian isolate of Turnip mosaic virus was determined by Sanger sequencing. After seven years of serial passaging by mechanical inoculation, the isolate was resequenced by RNA sequencing (RNA-Seq). Eighteen single nucleotide polymorphisms were identified between the isolates. Both isolates had 96% identity to isolate AUST10. ...[more]

PMID: 27856582

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