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Epigenetic engineering reveals a balance between histone modifications and transcription in kinetochore maintenance.


ABSTRACT: Centromeres consist of specialized centrochromatin containing CENP-A nucleosomes intermingled with H3 nucleosomes carrying transcription-associated modifications. We have designed a novel synthetic biology 'in situ epistasis' analysis in which H3 dimethylated on lysine 4 (H3K4me2) demethylase LSD2 plus synthetic modules with competing activities are simultaneously targeted to a synthetic alphoidtetO HAC centromere. This allows us to uncouple transcription from histone modifications at the centromere. Here, we report that H3K4me2 loss decreases centromeric transcription, CENP-A assembly and stability and causes spreading of H3K9me3 across the HAC, ultimately inactivating the centromere. Surprisingly, CENP-28/Eaf6-induced transcription of the alphoidtetO array associated with H4K12 acetylation does not rescue the phenotype, whereas p65-induced transcription associated with H3K9 acetylation does rescue. Thus mitotic transcription plus histone modifications including H3K9ac constitute the 'epigenetic landscape' allowing CENP-A assembly and centrochromatin maintenance. H3K4me2 is required for the transcription and H3K9ac may form a barrier to prevent heterochromatin spreading and kinetochore inactivation at human centromeres.

SUBMITTER: Molina O 

PROVIDER: S-EPMC5114538 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Epigenetic engineering reveals a balance between histone modifications and transcription in kinetochore maintenance.

Molina Oscar O   Vargiu Giulia G   Abad Maria Alba MA   Zhiteneva Alisa A   Jeyaprakash A Arockia AA   Masumoto Hiroshi H   Kouprina Natalay N   Larionov Vladimir V   Earnshaw William C WC  

Nature communications 20161114


Centromeres consist of specialized centrochromatin containing CENP-A nucleosomes intermingled with H3 nucleosomes carrying transcription-associated modifications. We have designed a novel synthetic biology 'in situ epistasis' analysis in which H3 dimethylated on lysine 4 (H3K4me2) demethylase LSD2 plus synthetic modules with competing activities are simultaneously targeted to a synthetic alphoid<sup>tetO</sup> HAC centromere. This allows us to uncouple transcription from histone modifications at  ...[more]

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