Ontology highlight
ABSTRACT:
SUBMITTER: Molina O
PROVIDER: S-EPMC5114538 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Molina Oscar O Vargiu Giulia G Abad Maria Alba MA Zhiteneva Alisa A Jeyaprakash A Arockia AA Masumoto Hiroshi H Kouprina Natalay N Larionov Vladimir V Earnshaw William C WC
Nature communications 20161114
Centromeres consist of specialized centrochromatin containing CENP-A nucleosomes intermingled with H3 nucleosomes carrying transcription-associated modifications. We have designed a novel synthetic biology 'in situ epistasis' analysis in which H3 dimethylated on lysine 4 (H3K4me2) demethylase LSD2 plus synthetic modules with competing activities are simultaneously targeted to a synthetic alphoid<sup>tetO</sup> HAC centromere. This allows us to uncouple transcription from histone modifications at ...[more]