Dataset Information

Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia.

ABSTRACT: A living cell interrogation platform based on nanochannel electroporation is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi-target analysis and stimulus-response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis-free nucleic acid analysis on rare or hard-to-transfect cells.

SUBMITTER: Zhao X

PROVIDER: S-EPMC5115302 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia.

Zhao Xi X Huang Xiaomeng X Wang Xinmei X Wu Yun Y Eisfeld Ann-Kathrin AK Schwind Sebastian S Gallego-Perez Daniel D Boukany Pouyan E PE Marcucci Guido I GI Lee Ly James LJ

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20150716 12

<b>A living cell interrogation platform based on nanochannel electroporation</b> is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi-target analysis and stimulus-response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis-free nucleic acid analysis on rare or hard-to-transfect cells. ...[more]

PMID: 27980918

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Project description:IntroductionAcute myeloid leukemia (AML) imposes significant burden on patients, their families, and the healthcare system. Published literature has reported many AML signs and symptoms, as well as their impact on patients. However, there are no publications on the experience of living with AML from the patient's perspective. In this study, we performed qualitative interviews with patients with AML to understand their experience.MethodsParticipants were recruited from the US and Japan. All patients were screened to assess eligibility, and were divided into four subgroups (i.e., newly-diagnosed, high-intensity chemotherapy; newly-diagnosed, low-intensity chemotherapy; relapse/refractory; and post-transplant). Patients were interviewed over the phone by a trained researcher and asked about their day-to-day experience with AML. Signs/symptoms and impacts were coded, analyzed using Atlas.ti software, and reported as frequencies, with the medians of patient-reported disturbance levels (0-10) computed for each symptom and impact.ResultsThe most commonly reported sign/symptom in the US was fatigue (95.7%), followed by bruising and weakness (both 78.3%), and in Japan, nausea (94.4%), followed by fatigue and headache (both 88.9%). The most commonly reported impact in the US was a decreased ability to maintain social/familial roles (91.3%), followed by anxiety and a decreased ability to function (both 87.0%), and most commonly reported in Japan was anxiety, a decreased ability to function, and remission uncertainty (94.4%).ConclusionAlthough the frequency of signs/symptoms and their level of disturbance varied between the US and Japan, there was remarkable consistency in the types of signs/symptoms and impacts reported across all patients. The consistency in the experience of the disease across patients suggests that measurement of AML experience can be achieved by using the same tool for most, if not all, of these patients.FundingAstellas Pharma Inc., Northbrook, IL, USA.

Dataset Information

Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia.

Publications

Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia.

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