Ontology highlight
ABSTRACT:
SUBMITTER: Gutierrez-Triana JA
PROVIDER: S-EPMC5117216 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Development (Cambridge, England) 20161005 22
DNA adenine methyltransferase identification (DamID) has emerged as an alternative method to profile protein-DNA interactions; however, critical issues limit its widespread applicability. Here, we present iDamIDseq, a protocol that improves specificity and sensitivity by inverting the steps DpnI-DpnII and adding steps that involve a phosphatase and exonuclease. To determine genome-wide protein-DNA interactions efficiently, we present the analysis tool iDEAR (iDamIDseq Enrichment Analysis with R) ...[more]