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Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction.

ABSTRACT: Burkholderia glumae (bacterial grain rot), Xanthomonas oryzae pv. oryzae (bacterial leaf blight), and Acidovorax avenae subsp. avenae (bacterial brown stripe) are major seedborne pathogens of rice. Based on the 16S and 23S rDNA sequences for A. avenae subsp. avenae and B. glumae, and transposase A gene sequence for X. oryzae pv. oryzae, three sets of primers had been designed to produce 402 bp for B. glumae, 490 bp for X. oryzae, and 290 bp for A. avenae subsp. avenae with the 63°C as an optimum annealing temperature. Samples collected from naturally infected fields were detected with two bacteria, B. glumae and A. avenae subsp. avenae but X. oryzae pv. oryzae was not detected. This assay can be used to identify pathogens directly from infected seeds, and will be an effective tool for the identification of the three pathogens in rice plants.

SUBMITTER: Kang IJ 

PROVIDER: S-EPMC5117867 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction.

Kang In Jeong IJ   Kang Mi-Hyung MH   Noh Tae-Hwan TH   Shim Hyeong Kwon HK   Shin Dong Bum DB   Heu Suggi S  

The plant pathology journal 20161201 6

<i>Burkholderia glumae</i> (bacterial grain rot), <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (bacterial leaf blight), and <i>Acidovorax avenae</i> subsp. <i>avenae</i> (bacterial brown stripe) are major seedborne pathogens of rice. Based on the 16S and 23S rDNA sequences for <i>A. avenae</i> subsp. <i>avenae</i> and <i>B. glumae</i>, and transposase A gene sequence for <i>X. oryzae</i> pv. <i>oryzae</i>, three sets of primers had been designed to produce 402 bp for <i>B. glumae</i>, 490 bp for  ...[more]

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