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Updated Multiplex PCR for Detection of All Six Plasmid-Mediated qnr Gene Families.


ABSTRACT: Plasmid-mediated qnr genes have been reported in bacteria worldwide and are widely associated with other relevant determinants of resistance in multiresistance plasmids. Here, we provide an update on a previously described multiplex PCR in order to detect all six qnr families (including qnrA, qnrS, qnrB, qnrC, qnrD, and qnrVC) described until now. The proposed method makes possible the screening of these genes, reducing cost and time, and it may demonstrate an underestimated prevalence of the latest variants described.

SUBMITTER: Kraychete GB 

PROVIDER: S-EPMC5119042 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Updated Multiplex PCR for Detection of All Six Plasmid-Mediated qnr Gene Families.

Kraychete Gabriela Bergiante GB   Botelho Larissa Alvarenga Batista LA   Campana Eloiza Helena EH   Picão Renata Cristina RC   Bonelli Raquel Regina RR  

Antimicrobial agents and chemotherapy 20161121 12


Plasmid-mediated qnr genes have been reported in bacteria worldwide and are widely associated with other relevant determinants of resistance in multiresistance plasmids. Here, we provide an update on a previously described multiplex PCR in order to detect all six qnr families (including qnrA, qnrS, qnrB, qnrC, qnrD, and qnrVC) described until now. The proposed method makes possible the screening of these genes, reducing cost and time, and it may demonstrate an underestimated prevalence of the la  ...[more]

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