Ontology highlight
ABSTRACT:
SUBMITTER: Rodriguez-Molina JB
PROVIDER: S-EPMC5122673 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Rodríguez-Molina Juan B JB Tseng Sandra C SC Simonett Shane P SP Taunton Jack J Ansari Aseem Z AZ
Molecular cell 20160728 3
During transcription initiation, the TFIIH-kinase Kin28/Cdk7 marks RNA polymerase II (Pol II) by phosphorylating the C-terminal domain (CTD) of its largest subunit. Here we describe a structure-guided chemical approach to covalently and specifically inactivate Kin28 kinase activity in vivo. This method of irreversible inactivation recapitulates both the lethal phenotype and the key molecular signatures that result from genetically disrupting Kin28 function in vivo. Inactivating Kin28 impacts pro ...[more]