Unknown

Dataset Information

0

Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells.

ABSTRACT: The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state.

SUBMITTER: Juan AH 

PROVIDER: S-EPMC5123747 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental c  ...[more]

Similar Datasets

Transcriptomics Distinct Roles of H3K27me2 and H3K27me3 States Unveiled By Fate Specification of Embryonic Stem Cells
2016-09-01 | GSE85717 | GEO
Unknown E2A regulates neural ectoderm fate specification in human embryonic stem cells.
| S-EPMC7758636 | biostudies-literature
Unknown Cholesterol-derived glucocorticoids control early fate specification in embryonic stem cells.
| S-EPMC4516691 | biostudies-literature
Unknown Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells.
| S-EPMC4388500 | biostudies-literature
Unknown Human stem cells from single blastomeres reveal pathways of embryonic or trophoblast fate specification.
| S-EPMC4712832 | biostudies-literature
Unknown Effects of 3D microwell culture on initial fate specification in human embryonic stem cells.
| S-EPMC4258881 | biostudies-literature
Unknown Transcriptional and epigenetic dynamics during specification of human embryonic stem cells.
| S-EPMC3709577 | biostudies-literature
Unknown Temporally restricted substrate interactions direct fate and specification of neural precursors derived from embryonic stem cells.
| S-EPMC1544174 | biostudies-literature
Unknown FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells.
| S-EPMC7565644 | biostudies-literature
Unknown Fate specification is spatially intermingled across planarian stem cells.
| S-EPMC10654723 | biostudies-literature