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Paper-Origami-Based Multiplexed Malaria Diagnostics from Whole Blood.

ABSTRACT: We demonstrate, for the first time, the multiplexed determination of microbial species from whole blood using the paper-folding technique of origami to enable the sequential steps of DNA extraction, loop-mediated isothermal amplification (LAMP), and array-based fluorescence detection. A low-cost handheld flashlight reveals the presence of the final DNA amplicon to the naked eye, providing a "sample-to-answer" diagnosis from a finger-prick volume of human blood, within 45?min, with minimal user intervention. To demonstrate the method, we showed the identification of three species of Plasmodium, analyzing 80?patient samples benchmarked against the gold-standard polymerase chain reaction (PCR) assay in an operator-blinded study. We also show that the test retains its diagnostic accuracy when using stored or fixed reference samples.

SUBMITTER: Xu G

PROVIDER: S-EPMC5132111 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Paper-Origami-Based Multiplexed Malaria Diagnostics from Whole Blood.

Xu Gaolian G Nolder Debbie D Reboud Julien J Oguike Mary C MC van Schalkwyk Donelly A DA Sutherland Colin J CJ Cooper Jonathan M JM

Angewandte Chemie (International ed. in English) 20160824 49

We demonstrate, for the first time, the multiplexed determination of microbial species from whole blood using the paper-folding technique of origami to enable the sequential steps of DNA extraction, loop-mediated isothermal amplification (LAMP), and array-based fluorescence detection. A low-cost handheld flashlight reveals the presence of the final DNA amplicon to the naked eye, providing a "sample-to-answer" diagnosis from a finger-prick volume of human blood, within 45 min, with minimal user i ...[more]

PMID: 27554333

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