An origami paper-based nanoformulated immunosensor detects picograms of VEGF-C per milliliter of blood.

ABSTRACT: Detecting vascular endothelial growth factor C (VEGF-C), a kind of tumor biomarker, is of significant clinical importance in evaluating the prognosis of patients with cancer. However, laboratory analyses are usually not suitable for point-of-care testing because they are expensive and time consuming. In response to these challenges, we fabricated an origami paper-based microfluidic electrochemical device. To improve the specificity of VEGF-C detection, nanocomposites, synthesized by new methylene blue (NMB), amino-functional single-walled carbon nanotubes (NH₂-SWCNTs), and gold nanoparticles (AuNPs), were used to modify the surface of working electrodes. Results of electrochemical detection showed that the immunosensor had excellent linearity, ranging from 0.01 to 100?ng?mL^-1 (R²?=?0.988), and the limit of detection was 10?pg?mL^-1. To confirm the high specificity of the device under real-world conditions, we evaluated the device using clinical serum samples from our hospital. The results demonstrated that the device had an excellent performance and could provide a platform for real-time detection of cancers.

SUBMITTER: Sun S 

PROVIDER: S-EPMC7838172 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature