Ontology highlight
ABSTRACT:
SUBMITTER: Hefzi H
PROVIDER: S-EPMC5132346 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Hefzi Hooman H Ang Kok Siong KS Hanscho Michael M Bordbar Aarash A Ruckerbauer David D Lakshmanan Meiyappan M Orellana Camila A CA Baycin-Hizal Deniz D Huang Yingxiang Y Ley Daniel D Martinez Veronica S VS Kyriakopoulos Sarantos S Jiménez Natalia E NE Zielinski Daniel C DC Quek Lake-Ee LE Wulff Tune T Arnsdorf Johnny J Li Shangzhong S Lee Jae Seong JS Paglia Giuseppe G Loira Nicolas N Spahn Philipp N PN Pedersen Lasse E LE Gutierrez Jahir M JM King Zachary A ZA Lund Anne Mathilde AM Nagarajan Harish H Thomas Alex A Abdel-Haleem Alyaa M AM Zanghellini Juergen J Kildegaard Helene F HF Voldborg Bjørn G BG Gerdtzen Ziomara P ZP Betenbaugh Michael J MJ Palsson Bernhard O BO Andersen Mikael R MR Nielsen Lars K LK Borth Nicole N Lee Dong-Yup DY Lewis Nathan E NE
Cell systems 20161101 5
Chinese hamster ovary (CHO) cells dominate biotherapeutic protein production and are widely used in mammalian cell line engineering research. To elucidate metabolic bottlenecks in protein production and to guide cell engineering and bioprocess optimization, we reconstructed the metabolic pathways in CHO and associated them with >1,700 genes in the Cricetulus griseus genome. The genome-scale metabolic model based on this reconstruction, iCHO1766, and cell-line-specific models for CHO-K1, CHO-S, a ...[more]