Ontology highlight
ABSTRACT:
SUBMITTER: MacDonald ML
PROVIDER: S-EPMC6430115 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
MacDonald Madolyn L ML Hamaker Nathaniel N Lee Kelvin H KH
AIChE journal. American Institute of Chemical Engineers 20180809 12
Complete, accurate genome assemblies are necessary to design targets for genetic engineering strategies. Successful gene knockdowns and knockouts in Chinese hamster ovary (CHO) cells may prevent the expression of difficult-to-remove host cell proteins (HCPs). HCPs, if not removed, can cause problems in stability, safety, and efficacy of the biotherapeutic. A significantly improved Chinese hamster (CH) reference genome was used to identify new knockout targets with similar predicted functions and ...[more]