Ontology highlight
ABSTRACT:
SUBMITTER: Montiel-Gonzalez MF
PROVIDER: S-EPMC5137428 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Montiel-González Maria Fernanda MF Vallecillo-Viejo Isabel C IC Rosenthal Joshua J C JJ
Nucleic acids research 20160823 21
Site-directed RNA editing (SDRE) is a strategy to precisely alter genetic information within mRNAs. By linking the catalytic domain of the RNA editing enzyme ADAR to an antisense guide RNA, specific adenosines can be converted to inosines, biological mimics for guanosine. Previously, we showed that a genetically encoded iteration of SDRE could target adenosines expressed in human cells, but not efficiently. Here we developed a reporter assay to quantify editing, and used it to improve our strate ...[more]