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Staphylococcus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing.

ABSTRACT: Staphylococcus aureus is the leading cause of wound and hospital-acquired infections worldwide. The emergence of S. aureus strains with resistance to multiple antibiotics requires the identification of bacterial virulence genes and the development of novel therapeutic strategies. Herein, bursa aurealis, a mariner-based transposon, was used for random mutagenesis and for the isolation of 10,325 S. aureus variants with defined insertion sites. By screening for loss-of-function mutants in a Caenorhabditis elegans killing assay, 71 S. aureus virulence genes were identified. Some of these genes are also required for S. aureus abscess formation in a murine infection model.

SUBMITTER: Bae T

PROVIDER: S-EPMC514475 | biostudies-literature | 2004 Aug

REPOSITORIES: biostudies-literature

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Staphylococcus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing.

Bae Taeok T Banger Alison K AK Wallace Adam A Glass Elizabeth M EM Aslund Fredrik F Schneewind Olaf O Missiakas Dominique M DM

Proceedings of the National Academy of Sciences of the United States of America 20040810 33

Staphylococcus aureus is the leading cause of wound and hospital-acquired infections worldwide. The emergence of S. aureus strains with resistance to multiple antibiotics requires the identification of bacterial virulence genes and the development of novel therapeutic strategies. Herein, bursa aurealis, a mariner-based transposon, was used for random mutagenesis and for the isolation of 10,325 S. aureus variants with defined insertion sites. By screening for loss-of-function mutants in a Caenorh ...[more]

PMID: 15304642

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