Colorectal cancers with microsatellite instability display mRNA expression signatures characteristic of increased immunogenicity.

ABSTRACT:

Background

Colorectal cancers displaying high-degree microsatellite instability (MSI-H) have an improved prognosis compared to microsatellite stable (MSS) cancers. The observation of pronounced lymphocytic infiltrates suggests that MSI-H cancers are inherently more immunogenic. We aimed to compare the gene expression profiles of MSI-H and MSS cancers to provide evidence for an activated immune response in the former.

Results

We analysed tissue from 133 colorectal cancer patients with full consent and Local Ethics Committee approval. Genomic DNA was analysed for microsatellite instability in BAT-26. High-quality RNA was used for microarray analysis on the Affymetrix HG-U133A chip. Data was analysed on GeneSpring software version 6.0. Confirmatory real-time RT-PCR was performe

SUBMITTER: Banerjea A 

PROVIDER: S-EPMC514528 | biostudies-literature | 2004 Aug

REPOSITORIES: biostudies-literature