Unknown

Dataset Information

0

Mouse retrovirus mediates porcine endogenous retrovirus transmission into human cells in long-term human-porcine chimeric mice.


ABSTRACT: Porcine endogenous retrovirus (PERV) is a potential pathogen in clinical xenotransplantation; transmission of PERV in vivo has been suggested in murine xenotransplantation models. We analyzed the transmission of PERV to human cells in vivo using a model in which immunodeficient NOD/SCID transgenic mice were transplanted with porcine and human lymphohematopoietic tissues. Our results demonstrate, we believe for the first time, that human and pig cells can coexist long-term (up to 25 weeks) without direct PERV infection of human cells. Despite the transplantation of porcine cells that did not produce human-tropic PERV, human cells from the chimeric mice were frequently found to contain PERV sequences. However, this transmission was due to the pseudotyping of PERV-C (a virus without human tropism) by xenotropic murine leukemia virus, rather than to de novo generation of human-tropic PERV. Thus, pseudotyping might account for the PERV transmission previously observed in mice. The absence of direct human cell infection following long-term in vivo coexistence with large numbers of porcine cells provides encouragement regarding the potential safety of using pigs that do not produce human-tropic PERV as source animals for transplantation to humans.

SUBMITTER: Yang YG 

PROVIDER: S-EPMC514590 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Mouse retrovirus mediates porcine endogenous retrovirus transmission into human cells in long-term human-porcine chimeric mice.

Yang Yong-Guang YG   Wood James C JC   Lan Ping P   Wilkinson Robert A RA   Sykes Megan M   Fishman Jay A JA   Patience Clive C  

The Journal of clinical investigation 20040901 5


Porcine endogenous retrovirus (PERV) is a potential pathogen in clinical xenotransplantation; transmission of PERV in vivo has been suggested in murine xenotransplantation models. We analyzed the transmission of PERV to human cells in vivo using a model in which immunodeficient NOD/SCID transgenic mice were transplanted with porcine and human lymphohematopoietic tissues. Our results demonstrate, we believe for the first time, that human and pig cells can coexist long-term (up to 25 weeks) withou  ...[more]

Similar Datasets

| S-EPMC5546926 | biostudies-literature
| S-EPMC2820912 | biostudies-literature
| S-EPMC5104465 | biostudies-literature
| S-EPMC135987 | biostudies-literature
| S-EPMC3126140 | biostudies-literature
| S-EPMC5629053 | biostudies-literature
| S-EPMC6769330 | biostudies-literature
| S-EPMC1472152 | biostudies-literature
| S-EPMC533951 | biostudies-literature
| S-EPMC140639 | biostudies-literature