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Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction.


ABSTRACT: Hsp70 chaperones assist de novo folding of newly synthesized proteins in all cells. In yeast, the specialized Hsp70 Ssb directly binds to ribosomes. The structural basis and functional mode of recruitment of Ssb to ribosomes is not understood. Here, we present the molecular details underlying ribosome binding of Ssb in Saccharomyces cerevisiae. This interaction is multifaceted, involving the co-chaperone RAC and two specific regions within Ssb characterized by positive charges. The C-terminus of Ssb mediates the key contact and a second attachment point is provided by a KRR-motif in the substrate binding domain. Strikingly, ribosome binding of Ssb is not essential. Autonomous ribosome attachment becomes necessary if RAC is absent, suggesting a dual mode of Ssb recruitment to nascent chains. We propose, that the multilayered ribosomal interaction allows positioning of Ssb in an optimal orientation to the tunnel exit guaranteeing an efficient nascent polypeptide interaction.

SUBMITTER: Hanebuth MA 

PROVIDER: S-EPMC5150220 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction.

Hanebuth Marie A MA   Kityk Roman R   Fries Sandra J SJ   Jain Alok A   Kriel Allison A   Albanese Veronique V   Frickey Tancred T   Peter Christine C   Mayer Matthias P MP   Frydman Judith J   Deuerling Elke E  

Nature communications 20161205


Hsp70 chaperones assist de novo folding of newly synthesized proteins in all cells. In yeast, the specialized Hsp70 Ssb directly binds to ribosomes. The structural basis and functional mode of recruitment of Ssb to ribosomes is not understood. Here, we present the molecular details underlying ribosome binding of Ssb in Saccharomyces cerevisiae. This interaction is multifaceted, involving the co-chaperone RAC and two specific regions within Ssb characterized by positive charges. The C-terminus of  ...[more]

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