Ontology highlight
ABSTRACT:
SUBMITTER: Rose JC
PROVIDER: S-EPMC5161653 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Rose John C JC Huang Po-Ssu PS Camp Nathan D ND Ye Jordan J Leidal Andrew M AM Goreshnik Inna I Trevillian Bridget M BM Dickinson Miles S MS Cunningham-Bryant Daniel D Debnath Jayanta J Baker David D Wolf-Yadlin Alejandro A Maly Dustin J DJ
Nature chemical biology 20161121 1
Synthetic protein switches controlled with user-defined inputs are powerful tools for studying and controlling dynamic cellular processes. To date, these approaches have relied primarily on intermolecular regulation. Here we report a computationally guided framework for engineering intramolecular regulation of protein function. We utilize this framework to develop chemically inducible activator of RAS (CIAR), a single-component RAS rheostat that directly activates endogenous RAS in response to a ...[more]