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A hairpin turn in a class II MHC-bound peptide orients residues outside the binding groove for T cell recognition.


ABSTRACT: T cells generally recognize peptide antigens bound to MHC proteins through contacts with residues found within or immediately flanking the seven- to nine-residue sequence accommodated in the MHC peptide-binding groove. However, some T cells require peptide residues outside this region for activation, the structural basis for which is unknown. Here, we have investigated a HIV Gag-specific T cell clone that requires an unusually long peptide antigen for activation. The crystal structure of a minimally antigenic 16-mer bound to HLA-DR1 shows that the peptide C-terminal region bends sharply into a hairpin turn as it exits the binding site, orienting peptide residues outside the MHC-binding region in position to interact with a T cell receptor. Peptide truncation and substitution studies show that both the hairpin turn and the extreme C-terminal residues are required for T cell activation. These results demonstrate a previously unrecognized mode of MHC-peptide-T cell receptor interaction.

SUBMITTER: Zavala-Ruiz Z 

PROVIDER: S-EPMC516560 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature

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A hairpin turn in a class II MHC-bound peptide orients residues outside the binding groove for T cell recognition.

Zavala-Ruiz Zarixia Z   Strug Iwona I   Walker Bruce D BD   Norris Philip J PJ   Stern Lawrence J LJ  

Proceedings of the National Academy of Sciences of the United States of America 20040826 36


T cells generally recognize peptide antigens bound to MHC proteins through contacts with residues found within or immediately flanking the seven- to nine-residue sequence accommodated in the MHC peptide-binding groove. However, some T cells require peptide residues outside this region for activation, the structural basis for which is unknown. Here, we have investigated a HIV Gag-specific T cell clone that requires an unusually long peptide antigen for activation. The crystal structure of a minim  ...[more]

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