Ontology highlight
ABSTRACT:
SUBMITTER: Shimizu M
PROVIDER: S-EPMC5167161 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Shimizu Masahiro M Noguchi Yasunori Y Sakiyama Yukari Y Kawakami Hironori H Katayama Tsutomu T Takada Shoji S
Proceedings of the National Academy of Sciences of the United States of America 20161129 50
Upon DNA replication initiation in Escherichia coli, the initiator protein DnaA forms higher-order complexes with the chromosomal origin oriC and a DNA-bending protein IHF. Although tertiary structures of DnaA and IHF have previously been elucidated, dynamic structures of oriC-DnaA-IHF complexes remain unknown. Here, combining computer simulations with biochemical assays, we obtained models at almost-atomic resolution for the central part of the oriC-DnaA-IHF complex. This complex can be divided ...[more]