Ontology highlight
ABSTRACT:
SUBMITTER: Kim J
PROVIDER: S-EPMC5167648 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Kim Jinsu J Poling-Skutvik Ryan R Trabuco João R C JR Kourentzi Katerina K Willson Richard C RC Conrad Jacinta C JC
The Analyst 20161201 1
Using microscopy and image analysis, we characterize binding of filamentous viral nanoparticles to a fibrous affinity matrix as models for reporter capture in a lateral flow assay (LFA). M13 bacteriophage (M13) displaying an in vivo-biotinylated peptide (AviTag) genetically fused to the M13 tail protein p3 are functionalized with fluorescent labels. We functionalize glass fiber LFA membranes with antibodies to M13, which primarily capture M13 on the major p8 coat proteins, or with avidin, which ...[more]