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ABSTRACT:
SUBMITTER: Adhikari M
PROVIDER: S-EPMC4666763 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Adhikari Meena M Strych Ulrich U Kim Jinsu J Goux Heather H Dhamane Sagar S Poongavanam Mohan-Vivekanandan MV Hagström Anna E V AE Kourentzi Katerina K Conrad Jacinta C JC Willson Richard C RC
Analytical chemistry 20151111 23
We introduce the modification of bacteriophage particles with aptamers for use as bioanalytical reporters, and demonstrate the use of these particles in ultrasensitive lateral flow assays. M13 phage displaying an in vivo biotinylatable peptide (AviTag) genetically fused to the phage tail protein pIII were used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (HRP) reporter enzymes covalently attached to the pVIII coat prot ...[more]