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Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged ?2-8-sialosides and high-throughput substrate specificity studies of ?2-8-sialidases.


ABSTRACT: para-Nitrophenol (pNP)-tagged ?2-8-linked sialosides containing different sialic acid forms were chemoenzymatically synthesized using an efficient one-pot three-enzyme ?2-8-sialylation system. The resulting compounds allowed high-throughput substrate specificity studies of the ?2-8-sialidase activity of a recombinant human cytosolic sialidase hNEU2 and various bacterial sialidases. The sialoside substrate profiles obtained can be used to guide the selection of suitable sialidases for sialylglycan analysis and for cell and tissue surface glycan modification. They can also be used to guide sialidase inhibitor design.

SUBMITTER: Tasnima N 

PROVIDER: S-EPMC5173422 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged α2-8-sialosides and high-throughput substrate specificity studies of α2-8-sialidases.

Tasnima Nova N   Yu Hai H   Li Yanhong Y   Santra Abhishek A   Chen Xi X  

Organic & biomolecular chemistry 20161201 1


para-Nitrophenol (pNP)-tagged α2-8-linked sialosides containing different sialic acid forms were chemoenzymatically synthesized using an efficient one-pot three-enzyme α2-8-sialylation system. The resulting compounds allowed high-throughput substrate specificity studies of the α2-8-sialidase activity of a recombinant human cytosolic sialidase hNEU2 and various bacterial sialidases. The sialoside substrate profiles obtained can be used to guide the selection of suitable sialidases for sialylglyca  ...[more]

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