Unknown

Dataset Information

0

NF-κB signalling and cell fate decisions in response to a short pulse of tumour necrosis factor.


ABSTRACT: In tissues and tumours, cell behaviours are regulated by multiple time-varying signals. While in the laboratory cells are often exposed to a stimulus for the duration of the experiment, in vivo exposures may be much shorter. In this study, we monitored NF-κB and caspase signalling in human cancer cells treated with a short pulse of Tumour Necrosis Factor (TNF). TNF is an inflammatory cytokine that can induce both the pro-survival NF-κB-driven gene transcription pathway and the pro-apoptotic caspase pathway. We find that a few seconds of exposure to TNF is sufficient to activate the NF-κB pathway in HeLa cells and induce apoptotic cell death in both HeLa and Kym-1 cells. Strikingly, a 1-min pulse of TNF can be more effective at killing than a 1-hour pulse, indicating that in addition to TNF concentration, duration of exposure also coordinates cell fate decisions.

SUBMITTER: Lee RE 

PROVIDER: S-EPMC5177917 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC2413189 | biostudies-literature
| S-EPMC4135562 | biostudies-literature
2017-12-15 | GSE107668 | GEO
| S-EPMC6558032 | biostudies-literature
| S-EPMC7020041 | biostudies-literature
| S-EPMC6627693 | biostudies-literature
| S-EPMC5598316 | biostudies-literature
| S-EPMC7521273 | biostudies-literature
| S-EPMC1224034 | biostudies-other
| S-EPMC6761572 | biostudies-literature