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Riches in RAGs: Revealing the V(D)J Recombinase through High-Resolution Structures.

ABSTRACT: Development of the adaptive immune system is dependent on V(D)J recombination, which forms functional antigen receptor genes through rearrangement of component gene segments. The V(D)J recombinase, comprising recombination-activating proteins RAG1 and RAG2, guides the initial DNA cleavage events to the recombination signal sequence (RSS), which flanks each gene segment. Although the enzymatic steps for RAG-mediated endonucleolytic activity were established over two decades ago, only recently have high-resolution structural studies of the catalytically active core regions of the RAG proteins shed light on conformational requirements for the reaction. While outstanding questions remain, we have a clearer picture of how RAG proteins function in generating the diverse repertoires of antigen receptors, the underlying foundation of the adaptive immune system.

SUBMITTER: Rodgers KK

PROVIDER: S-EPMC5182142 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Riches in RAGs: Revealing the V(D)J Recombinase through High-Resolution Structures.

Rodgers Karla K KK

Trends in biochemical sciences 20161105 1

Development of the adaptive immune system is dependent on V(D)J recombination, which forms functional antigen receptor genes through rearrangement of component gene segments. The V(D)J recombinase, comprising recombination-activating proteins RAG1 and RAG2, guides the initial DNA cleavage events to the recombination signal sequence (RSS), which flanks each gene segment. Although the enzymatic steps for RAG-mediated endonucleolytic activity were established over two decades ago, only recently hav ...[more]

PMID: 27825771

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