Unknown

Dataset Information

0

Characterizing polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB+1.


ABSTRACT: The ubiquitin mutant UBB+1 has been identified as a hallmark of neurodegenerative diseases. In this study, we characterize polyubiquitinated forms of UBB+1 in vitro and from patient samples. The ability of UBB+1 to be readily ubiquitinated by several E2 enzymes provided a mechanism for the controlled synthesis and purification of defined conjugates. This allowed us to utilize polyUb-UBB+1 conjugates for biochemical assays, as well as solution NMR. Coupled with our immunoassay for detection of ubiquitinated forms of UBB+1 in patient blood samples, we gain a clearer picture of the molecular mechanisms underlying neurodegenerative diseases.

SUBMITTER: Chojnacki M 

PROVIDER: S-EPMC5182158 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Characterizing polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB<sup>+1</sup>.

Chojnacki Michal M   Zhang Daoning D   Talarowska Monika M   Gałecki Piotr P   Szemraj Janusz J   Fushman David D   Nakasone Mark A MA  

FEBS letters 20161122 24


The ubiquitin mutant UBB<sup>+1</sup> has been identified as a hallmark of neurodegenerative diseases. In this study, we characterize polyubiquitinated forms of UBB<sup>+1</sup> in vitro and from patient samples. The ability of UBB<sup>+1</sup> to be readily ubiquitinated by several E2 enzymes provided a mechanism for the controlled synthesis and purification of defined conjugates. This allowed us to utilize polyUb-UBB<sup>+1</sup> conjugates for biochemical assays, as well as solution NMR. Coup  ...[more]

Similar Datasets

| S-EPMC8032880 | biostudies-literature
| S-EPMC6008557 | biostudies-literature
| S-EPMC7066184 | biostudies-literature
| S-EPMC8610117 | biostudies-literature
| S-EPMC4062464 | biostudies-literature
| S-EPMC3769649 | biostudies-literature
2020-03-19 | PXD014318 | Pride
| S-EPMC3816408 | biostudies-literature
| S-EPMC3338923 | biostudies-literature
| S-EPMC5343084 | biostudies-literature