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SKN-1-independent transcriptional activation of glutathione S-transferase 4 (GST-4) by EGF signaling.

ABSTRACT: In C. elegans research, transcriptional activation of glutathione S-transferase 4 (gst-4) is often used as a read-out for SKN-1 activity. While many heed an assumed non-exclusivity of the GFP reporter signal driven by the gst-4 promoter to SKN-1, this is also often ignored. We here show that gst-4 can also be transcriptionally activated by EOR-1, a transcription factor mediating effects of the epidermal growth factor (EGF) pathway. Along with enhancing exogenous oxidative stress tolerance, EOR-1 inde-pendently of SKN-1 increases gst-4 transcription in response to augmented EGF signaling. Our findings caution researchers within the C. elegans community to always rely on sufficient experimental controls when assaying SKN-1 transcriptional activity with a gst-4_p::gfp reporter, such as SKN-1 loss-of-function mutants and/or additional target genes next to gst-4.

SUBMITTER: Detienne G

PROVIDER: S-EPMC5190145 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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SKN-1-independent transcriptional activation of glutathione S-transferase 4 (GST-4) by EGF signaling.

Detienne Giel G Van de Walle Pieter P De Haes Wouter W Schoofs Liliane L Temmerman Liesbet L

Worm 20160831 4

In C. elegans research, transcriptional activation of glutathione S-transferase 4 (gst-4) is often used as a read-out for SKN-1 activity. While many heed an assumed non-exclusivity of the GFP reporter signal driven by the gst-4 promoter to SKN-1, this is also often ignored. We here show that gst-4 can also be transcriptionally activated by EOR-1, a transcription factor mediating effects of the epidermal growth factor (EGF) pathway. Along with enhancing exogenous oxida ...[more]

PMID: 28090393

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