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CTX-M-190, a Novel ?-Lactamase Resistant to Tazobactam and Sulbactam, Identified in an Escherichia coli Clinical Isolate.

ABSTRACT: A novel ?-lactamase, CTX-M-190, derived from CTX-M-55 by a single substitution of Ser for Thr at position 133 (Ser133Thr), was identified in a natural Escherichia coli clinical isolate. CTX-M-190 exhibited potent hydrolytic activity against cefotaxime, with a kcat/Km ratio of 14.5 ?M-1 s-1, and was highly resistant to inhibition by the ?-lactamase inhibitors tazobactam and sulbactam, whose 50% inhibitory concentrations were 77- and 55-fold higher, respectively, for CTX-M-190 than for CTX-M-55. blaCTX-M-190 was located within the genetic platform ISEcp1-blaCTX-M-orf477, which was harbored by a 70-kb IncI1 plasmid.

SUBMITTER: Shen Z

PROVIDER: S-EPMC5192109 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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CTX-M-190, a Novel β-Lactamase Resistant to Tazobactam and Sulbactam, Identified in an Escherichia coli Clinical Isolate.

Shen Zhen Z Ding Baixing B Bi Yingmin Y Wu Shi S Xu Su S Xu Xiaogang X Guo Qinglan Q Wang Minggui M

Antimicrobial agents and chemotherapy 20161227 1

A novel β-lactamase, CTX-M-190, derived from CTX-M-55 by a single substitution of Ser for Thr at position 133 (Ser133Thr), was identified in a natural Escherichia coli clinical isolate. CTX-M-190 exhibited potent hydrolytic activity against cefotaxime, with a kcat/Km ratio of 14.5 μM-1 s-1, and was highly resistant to inhibition by the β-lactamase inhibitors tazobactam and sulbactam, whose 50% inhibitory concentrations were 77- and 55-fold higher, resp ...[more]

PMID: 27821452

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