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NDM-12, a novel New Delhi metallo-?-lactamase variant from a carbapenem-resistant Escherichia coli clinical isolate in Nepal.

ABSTRACT: A novel New Delhi metallo-?-lactamase variant, NDM-12, was identified in a carbapenem-resistant Escherichia coli clinical isolate obtained from a urine sample from a patient in Nepal. NDM-12 differed from NDM-1 by two amino acid substitutions (M154L and G222D). The enzymatic activities of NDM-12 against ?-lactams were similar to those of NDM-1, although NDM-12 showed lower kcat/Km ratios for all ?-lactams tested except doripenem. The blaNDM-12 gene was located in a plasmid of 160 kb.

SUBMITTER: Tada T

PROVIDER: S-EPMC4187903 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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NDM-12, a novel New Delhi metallo-β-lactamase variant from a carbapenem-resistant Escherichia coli clinical isolate in Nepal.

Tada Tatsuya T Shrestha Basudha B Miyoshi-Akiyama Tohru T Shimada Kayo K Ohara Hiroshi H Kirikae Teruo T Pokhrel Bharat M BM

Antimicrobial agents and chemotherapy 20140804 10

A novel New Delhi metallo-β-lactamase variant, NDM-12, was identified in a carbapenem-resistant Escherichia coli clinical isolate obtained from a urine sample from a patient in Nepal. NDM-12 differed from NDM-1 by two amino acid substitutions (M154L and G222D). The enzymatic activities of NDM-12 against β-lactams were similar to those of NDM-1, although NDM-12 showed lower kcat/Km ratios for all β-lactams tested except doripenem. The blaNDM-12 gene was located in a plasmid of 160 kb. ...[more]

PMID: 25092693

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