Ontology highlight
ABSTRACT:
SUBMITTER: Sun J
PROVIDER: S-EPMC5192969 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Sun Jingchuan J Yuan Zuanning Z Bai Lin L Li Huilin H
Protein science : a publication of the Protein Society 20160914 1
DNA replication in Eukaryotes is a highly dynamic process that involves several dozens of proteins. Some of these proteins form stable complexes that are amenable to high-resolution structure determination by cryo-EM, thanks to the recent advent of the direct electron detector and powerful image analysis algorithm. But many of these proteins associate only transiently and flexibly, precluding traditional biochemical purification. We found that direct mixing of the component proteins followed by ...[more]