Ontology highlight
ABSTRACT:
SUBMITTER: Abid Ali F
PROVIDER: S-EPMC5740162 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Abid Ali Ferdos F Douglas Max E ME Locke Julia J Pye Valerie E VE Nans Andrea A Diffley John F X JFX Costa Alessandro A
Nature communications 20171221 1
Eukaryotic origins of replication are licensed upon loading of the MCM helicase motor onto DNA. ATP hydrolysis by MCM is required for loading and the post-catalytic MCM is an inactive double hexamer that encircles duplex DNA. Origin firing depends on MCM engagement of Cdc45 and GINS to form the CMG holo-helicase. CMG assembly requires several steps including MCM phosphorylation by DDK. To understand origin activation, here we have determined the cryo-EM structures of DNA-bound MCM, either unmodi ...[more]