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Covalently circularized nanodiscs for studying membrane proteins and viral entry.


ABSTRACT: We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter sizes and tunable shapes. Reconstitution into cNDs enhanced the quality of nuclear magnetic resonance spectra for both VDAC-1, a ?-barrel membrane protein, and the G-protein-coupled receptor NTR1, an ?-helical membrane protein. In addition, we used cNDs to visualize how simple, nonenveloped viruses translocate their genomes across membranes to initiate infection.

SUBMITTER: Nasr ML 

PROVIDER: S-EPMC5199620 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Covalently circularized nanodiscs for studying membrane proteins and viral entry.

Nasr Mahmoud L ML   Baptista Diego D   Strauss Mike M   Sun Zhen-Yu J ZJ   Grigoriu Simina S   Huser Sonja S   Plückthun Andreas A   Hagn Franz F   Walz Thomas T   Hogle James M JM   Wagner Gerhard G  

Nature methods 20161121 1


We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter sizes and tunable shapes. Reconstitution into cNDs enhanced the quality of nuclear magnetic resonance spectra for both VDAC-1, a β-barrel membrane protein, and the G-protein-coupled receptor NTR1, an α-helical membrane protein. In addition, we used cNDs to visualize how simple, nonenveloped viruses translocate their genomes across membranes to initiate infe  ...[more]

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