Ontology highlight
ABSTRACT:
SUBMITTER: Stevens AJ
PROVIDER: S-EPMC5214457 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Stevens Aaron J AJ Kennedy Martin A MA
PloS one 20170104 1
The promoter region of the imprinted gene MEST contains several motifs capable of forming G-quadruplex (G4) structures, which appear to contribute to consistent allelic dropout during polymerase chain reaction (PCR) analysis of this region. Here, we extend our previous analysis of MEST G4 structures by applying fluorescent footprinting techniques to assess non B-DNA structure and topology in dsDNA from the full MEST promoter region, under conditions that mimic PCR. We demonstrate that the buffer ...[more]