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Quantitative Formation of Monomeric G-Quadruplex DNA from Multimeric Structures of c-Myc Promoter Sequence.

ABSTRACT: G-Quadruplex (G4)-forming DNA sequences have a tendency to form stable multimeric structures. This can be problematic for studies with synthetic oligodeoxynucleotides. Herein, we describe a method that quantitatively converts multimeric intermolecular structures of the Pu27 sequence from the c-myc promoter into the desired monomeric G4 by alkaline treatment and refolding.

SUBMITTER: Rauser V

PROVIDER: S-EPMC7496815 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Quantitative Formation of Monomeric G-Quadruplex DNA from Multimeric Structures of c-Myc Promoter Sequence.

Rauser Valerie V Weinhold Elmar E

Chembiochem : a European journal of chemical biology 20200511 17

G-Quadruplex (G4)-forming DNA sequences have a tendency to form stable multimeric structures. This can be problematic for studies with synthetic oligodeoxynucleotides. Herein, we describe a method that quantitatively converts multimeric intermolecular structures of the Pu27 sequence from the c-myc promoter into the desired monomeric G4 by alkaline treatment and refolding. ...[more]

PMID: 32267052

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Project description:This SuperSeries is composed of the SubSeries listed below.

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