Dataset Information

Metabolomic Profiling in Individuals with a Failing Kidney Allograft.

ABSTRACT:

Background

Alteration of certain metabolites may play a role in the pathophysiology of renal allograft disease.

Methods

To explore metabolomic abnormalities in individuals with a failing kidney allograft, we analyzed by liquid chromatography-mass spectrometry (LC-MS/MS; for ex vivo profiling of serum and urine) and two dimensional correlated spectroscopy (2D COSY; for in vivo study of the kidney graft) 40 subjects with varying degrees of chronic allograft dysfunction stratified by tertiles of glomerular filtration rate (GFR; T1, T2, T3). Ten healthy non-allograft individuals were chosen as controls.

Results

LC-MS/MS analysis revealed a dose-response association between GFR and serum concentration of tryptophan, glutamine, dimethylarginine isomers (asymmetric [A]DMA and symmetric [S]DMA) and short-chain acylcarnitines (C4 and C12), (test for trend: T1-T3 = p<0.05; p = 0.01; p<0.001; p = 0.01; p = 0.01; p<0.05, respectively). The same association was found between GFR and urinary levels of histidine, DOPA, dopamine, carnosine, SDMA and ADMA (test for trend: T1-T3 = p<0.05; p<0.01; p = 0.001; p<0.05; p = 0.001; p<0.001; p<0.01, respectively). In vivo 2D COSY of the kidney allograft revealed significant reduction in the parenchymal content of choline, creatine, taurine and threonine (all: p<0.05) in individuals with lower GFR levels.

Conclusions

We report an association between renal function and altered metabolomic profile in renal transplant individuals with different degrees of kidney graft function.

SUBMITTER: Bassi R

PROVIDER: S-EPMC5214547 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Metabolomic Profiling in Individuals with a Failing Kidney Allograft.

Bassi Roberto R Niewczas Monika A MA Biancone Luigi L Bussolino Stefania S Merugumala Sai S Tezza Sara S D'Addio Francesca F Ben Nasr Moufida M Valderrama-Vasquez Alessandro A Usuelli Vera V De Zan Valentina V El Essawy Basset B Venturini Massimo M Secchi Antonio A De Cobelli Francesco F Lin Alexander A Chandraker Anil A Fiorina Paolo P

PloS one 20170104 1

<h4>Background</h4>Alteration of certain metabolites may play a role in the pathophysiology of renal allograft disease.<h4>Methods</h4>To explore metabolomic abnormalities in individuals with a failing kidney allograft, we analyzed by liquid chromatography-mass spectrometry (LC-MS/MS; for ex vivo profiling of serum and urine) and two dimensional correlated spectroscopy (2D COSY; for in vivo study of the kidney graft) 40 subjects with varying degrees of chronic allograft dysfunction stratified by ...[more]

PMID: 28052095

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Dataset Information

Metabolomic Profiling in Individuals with a Failing Kidney Allograft.

Background

Methods

Results

Conclusions

Publications

Metabolomic Profiling in Individuals with a Failing Kidney Allograft.

Similar Datasets

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