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Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting.

ABSTRACT: Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.

SUBMITTER: Atkin-Smith GK

PROVIDER: S-EPMC5216387 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting.

Atkin-Smith Georgia K GK Paone Stephanie S Zanker Damien J DJ Duan Mubing M Phan Than K TK Chen Weisan W Hulett Mark D MD Poon Ivan K H IK

Scientific reports 20170106

Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-der ...[more]

PMID: 28057919

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| S-EPMC5575905 | biostudies-literature

Isolation of human skeletal muscle precursor cells by fluorescence-activated cell sorting.

Project description:Novel fluorescence-activated cell sorting (FACS) strategies to prospectively purify functionally distinct cell populations from the human myofiber-associated (hMFA) cell compartment, including human Skeletal Muscle Precursor cells (hSMPs): HSMPs, identified as CD45-Mac1-GlyA-CD31-CD34-CD56intITGA7hi hMFA cells, are highly enriched for cells expressing the satellite cell marker PAX7 and show efficient myogenic and lack adipogenic capacity. CD45-CD11b-GlyA-CD31-CD34+ hMFA cells (CD34+ cells) do not express PAX7, lack myogenic and exhibit adipogenic activity. We used Affymetrix Human Genome U133 Plus 2.0 microarrays to gain deeper insights into the molecular underpinnings functionally and phenotypically discrete human myofiber-associated cell subsets.

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Isolation of human skeletal muscle precursor cells by fluorescence-activated cell sorting.

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