Isolation of lightning-competent soil bacteria.
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ABSTRACT:
SUBMITTER: Ceremonie H
PROVIDER: S-EPMC522088 | biostudies-literature | 2004 Oct
REPOSITORIES: biostudies-literature
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Isolation of lightning-competent soil bacteria.
Cérémonie Hélène H Buret François F Simonet Pascal P Vogel Timothy M TM
Applied and environmental microbiology 20041001 10
Artificial transformation is typically performed in the laboratory by using either a chemical (CaCl(2)) or an electrical (electroporation) method. However, laboratory-scale lightning has been shown recently to electrotransform Escherichia coli strain DH10B in soil. In this paper, we report on the isolation of two "lightning-competent" soil bacteria after direct electroporation of the Nycodenz bacterial ring extracted from prairie soil in the presence of the pBHCRec plasmid (Tc(r), Sp(r), Sm(r)). ...[more]
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