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PET imaging of tenascin-C with a radiolabeled single-stranded DNA aptamer.


ABSTRACT: UNLABELLED:Tenascin-C is an extracellular matrix glycoprotein that is expressed by injured tissues and by various cancers. Recent publications showed that tenascin-C expression by cancer lesions predicts tumor growth, metastasis, and angiogenesis, suggesting tenascin-C as a potential therapeutic target. Currently there is no noninvasive method to determine tumoral tenascin-C expression in vivo. To address the need for an agent to image and quantify tenascin-C, we report the development of a radioactive PET tracer based on a tenascin-C-specific single-stranded DNA aptamer (tenascin-C aptamer). METHODS:Tenascin-C aptamer was radiolabeled with (18)F and (64)Cu. PET imaging studies for the evaluation of tumor uptake and pharmacokinetics of tenascin-C aptamer were performed in comparison to a nonspecific scrambled aptamer (Sc aptamer). RESULTS:The labeled tenascin-C aptamer provided clear visualization of tenascin-C-positive but not tenascin-C-negative tumors. The uptake of tenascin-C aptamer was significantly higher than that of Sc aptamer in tenascin-C-positive tumors. The labeled tenascin-C aptamer had fast clearance from the blood and other nonspecific organs through the kidneys, resulting in high tumor contrast. CONCLUSION:Our data suggest that suitably labeled tenascin-C aptamer can be used as a PET tracer to image tumor expression of tenascin-C with a high tumor-to-background ratio and might provide insightful and personalized medical data that will help determine appropriate treatment and monitoring.

SUBMITTER: Jacobson O 

PROVIDER: S-EPMC5226408 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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PET imaging of tenascin-C with a radiolabeled single-stranded DNA aptamer.

Jacobson Orit O   Yan Xuefeng X   Niu Gang G   Weiss Ido D ID   Ma Ying Y   Szajek Lawrence P LP   Shen Baozhong B   Kiesewetter Dale O DO   Chen Xiaoyuan X  

Journal of nuclear medicine : official publication, Society of Nuclear Medicine 20150219 4


<h4>Unlabelled</h4>Tenascin-C is an extracellular matrix glycoprotein that is expressed by injured tissues and by various cancers. Recent publications showed that tenascin-C expression by cancer lesions predicts tumor growth, metastasis, and angiogenesis, suggesting tenascin-C as a potential therapeutic target. Currently there is no noninvasive method to determine tumoral tenascin-C expression in vivo. To address the need for an agent to image and quantify tenascin-C, we report the development o  ...[more]

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