Ontology highlight
ABSTRACT:
SUBMITTER: Ruigrok HJ
PROVIDER: S-EPMC5232350 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Ruigrok Hermanus Johannes HJ Shahid Guillaume G Goudeau Bertrand B Poulletier de Gannes Florence F Poque-Haro Emmanuelle E Hurtier Annabelle A Lagroye Isabelle I Vacher Pierre P Arbault Stéphane S Sojic Neso N Veyret Bernard B Percherancier Yann Y
Biophysical journal 20170101 1
Multiplexed bioluminescence resonance energy transfer (BRET) assays were developed to monitor the activation of several functional transient receptor potential (TRP) channels in live cells and in real time. We probed both TRPV1 intramolecular rearrangements and its interaction with Calmodulin (CaM) under activation by chemical agonists and temperature. Our BRET study also confirmed that: (1) capsaicin and heat promoted distinct transitions, independently coupled to channel gating, and that (2) T ...[more]