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Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells.

ABSTRACT: Hematopoietic stem cells (HSCs) are rare cells that generate all the various types of blood and immune cells. High-quality transcriptome data have enabled the identification of significant genes for HSCs. However, most genes are expressed in various forms by alternative splicing (AS), extending transcriptome complexity. Here, we delineate AS to determine which isoforms are expressed in mouse HSCs. Our analysis of microarray and RNA-sequencing data includes differential expression of splicing factors that may regulate AS, and a complete map of splicing isoforms. Multiple types of isoforms for known HSC genes and unannotated splicing that may alter gene function are presented. Transcriptome-wide identification of genes and their respective isoforms in mouse HSCs will open another dimension for adult stem cells.

SUBMITTER: Goldstein O

PROVIDER: S-EPMC5233452 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells.

Goldstein Oron O Meyer Karin K Greenshpan Yariv Y Bujanover Nir N Feigin Mili M Ner-Gaon Hadas H Shay Tal T Gazit Roi R

Stem cell reports 20161229 1

Hematopoietic stem cells (HSCs) are rare cells that generate all the various types of blood and immune cells. High-quality transcriptome data have enabled the identification of significant genes for HSCs. However, most genes are expressed in various forms by alternative splicing (AS), extending transcriptome complexity. Here, we delineate AS to determine which isoforms are expressed in mouse HSCs. Our analysis of microarray and RNA-sequencing data includes differential expression of splicing fac ...[more]

PMID: 28041879

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