Ontology highlight
ABSTRACT:
SUBMITTER: Cheng B
PROVIDER: S-EPMC5235945 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Cheng Bokun B Zhou Qingxuan Q Weng Liwei L Leszyk John D JD Greenberg Marc M MM Tse-Dinh Yuk-Ching YC
FEBS letters 20161220 1
Topoisomerases catalyze changes in DNA topology by directing the movement of DNA strands through consecutive cleavage-rejoining reactions of the DNA backbone. We describe the use of a phenylselenyl-modified thymidine incorporated into a specific position of a partially unwound DNA substrate in crosslinking studies of Escherichia coli topoisomerase I to gain new insights into its catalytic mechanism. Crosslinking of the phenylselenyl-modified thymidine to the topoisomerase protein was achieved by ...[more]