Dataset Information

Characterization of 16 microsatellite markers for the Oreinotinus clade of Viburnum (Adoxaceae).

ABSTRACT: PREMISE OF THE STUDY:Microsatellite loci were isolated from four species of Viburnum (Adoxaceae) to study population structure and assess species boundaries among morphologically similar South American Viburnum species of the Oreinotinus clade. METHODS AND RESULTS:Using a microsatellite-enriched library and mining next-generation sequence data, 16 microsatellites were developed. Each locus was tested on two populations of V. triphyllum and one population of V. pichinchense. For nuclear loci, one to 13 alleles were recovered, expected heterozygosity ranged from 0 to 0.8975, Simpson diversity index ranged from 0.0167 to 1.000, and Shannon diversity index ranged from 0 to 2.3670 in a given population. For the mitochondrial locus, three to six alleles were recovered and unbiased haploid diversity values ranged from 0.756 to 0.853 in a given population. CONCLUSIONS:The 16 microsatellite loci developed for the Oreinotinus clade (Viburnum, Adoxaceae) will inform investigations of population structure and species boundaries within this group.

SUBMITTER: Barish S

PROVIDER: S-EPMC5238702 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Characterization of 16 microsatellite markers for the <i>Oreinotinus</i> clade of <i>Viburnum</i> (Adoxaceae).

Barish Syndi S Arakaki Mónica M Edwards Erika J EJ Donoghue Michael J MJ Clement Wendy L WL

Applications in plant sciences 20161208 12

<h4>Premise of the study</h4>Microsatellite loci were isolated from four species of <i>Viburnum</i> (Adoxaceae) to study population structure and assess species boundaries among morphologically similar South American <i>Viburnum</i> species of the <i>Oreinotinus</i> clade.<h4>Methods and results</h4>Using a microsatellite-enriched library and mining next-generation sequence data, 16 microsatellites were developed. Each locus was tested on two populations of <i>V. triphyllum</i> and one populatio ...[more]

PMID: 28101437

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Project description:Background and aimsEnlarged sterile flowers on the periphery of inflorescences increase the attractiveness of floral displays, and previous studies have generally demonstrated that these have positive effects on insect visitation and/or reproductive success. However, experiments have not specifically been designed to examine the benefits of sterile flowers under conditions that reflect the early stages in their evolution, i.e. when plants that produce sterile flowers are at low frequency.MethodsOver three years, three experiments were performed in natural populations of Viburnum lantanoides, which produces sterile marginal flowers (SMFs). The first experiment established that fruit production in V. lantanoides increases with the receipt of outcross pollen. The second tested the role of SMFs under extant conditions, comparing fruit production in two populations composed entirely of intact plants or entirely of plants with the SMFs removed. The third was designed to mimic the presumed context in which SMFs first evolved; here, SMFs were removed from all but a few plants in a population, and rates of insect visitation and fruit set were compared between plants with intact and denuded SMFs.Key resultsIn comparing whole populations, the presence of SMFs nearly doubled fruit set. Under simulated 'ancestral' conditions within a population, plants with intact SMFs received double the insect visits and produced significantly more fruits than denuded plants. There was no significant effect of the number of inflorescences or fertile flowers on insect visitation or fruit set, indicating that the presence of SMFs accounted for these differences.ConclusionsThe presence of SMFs significantly increased pollinator attraction and female reproductive success both in contemporary and simulated ancestral contexts, indicating that stabilizing selection is responsible for their maintenance, and directional selection likely drove their evolution when they first appeared. This study demonstrates a novel approach to incorporating historically relevant scenarios into experimental studies of floral evolution.

Dataset Information

Characterization of 16 microsatellite markers for the Oreinotinus clade of Viburnum (Adoxaceae).

Publications

Characterization of 16 microsatellite markers for the <i>Oreinotinus</i> clade of <i>Viburnum</i> (Adoxaceae).

Similar Datasets

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