Unknown

Dataset Information

0

Genomic Nucleosome Organization Reconstituted with Pure Proteins.


ABSTRACT: Chromatin remodelers regulate genes by organizing nucleosomes around promoters, but their individual contributions are obfuscated by the complex in vivo milieu of factor redundancy and indirect effects. Genome-wide reconstitution of promoter nucleosome organization with purified proteins resolves this problem and is therefore a critical goal. Here, we reconstitute four stages of nucleosome architecture using purified components: yeast genomic DNA, histones, sequence-specific Abf1/Reb1, and remodelers RSC, ISW2, INO80, and ISW1a. We identify direct, specific, and sufficient contributions that in vivo observations validate. First, RSC clears promoters by translating poly(dA:dT) into directional nucleosome removal. Second, partial redundancy is recapitulated where INO80 alone, or ISW2 at Abf1/Reb1sites, positions +1 nucleosomes. Third, INO80 and ISW2 each align downstream nucleosomal arrays. Fourth, ISW1a tightens the spacing to canonical repeat lengths. Such a minimal set of rules and proteins establishes core mechanisms by which promoter chromatin architecture arises through a blend of redundancy and specialization.

SUBMITTER: Krietenstein N 

PROVIDER: S-EPMC5240917 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Genomic Nucleosome Organization Reconstituted with Pure Proteins.

Krietenstein Nils N   Wal Megha M   Watanabe Shinya S   Park Bongsoo B   Peterson Craig L CL   Pugh B Franklin BF   Korber Philipp P  

Cell 20161001 3


Chromatin remodelers regulate genes by organizing nucleosomes around promoters, but their individual contributions are obfuscated by the complex in vivo milieu of factor redundancy and indirect effects. Genome-wide reconstitution of promoter nucleosome organization with purified proteins resolves this problem and is therefore a critical goal. Here, we reconstitute four stages of nucleosome architecture using purified components: yeast genomic DNA, histones, sequence-specific Abf1/Reb1, and remod  ...[more]

Similar Datasets

2016-08-14 | GSE72106 | GEO
2016-08-14 | E-GEOD-72106 | biostudies-arrayexpress
| S-EPMC4852979 | biostudies-literature
| EMPIAR-10513 | biostudies-other
| S-EPMC6028132 | biostudies-literature
| S-EPMC9481575 | biostudies-literature
| EMPIAR-10748 | biostudies-other
| EMPIAR-10749 | biostudies-other
| S-EPMC3446672 | biostudies-literature
| S-EPMC2735122 | biostudies-literature