IFN-? regulates human dental pulp stem cells behavior via NF-?B and MAPK signaling.

ABSTRACT: During caries, dental pulp expresses a range of pro-inflammatory cytokines in response to the infectious challenge. Interferon gamma (IFN-?) is a dimerized soluble cytokine, which is critical for immune responses. Previous study has demonstrated that IFN-? at relative high concentration (100?ng/mL) treatment improved the impaired dentinogenic and immunosuppressive regulatory functions of disease-derived dental pulp stem cells (DPSCs). However, little is known about the regulatory effects of IFN-? at relative low concentration on healthy DPSC behavior (including proliferation, migration, and multiple-potential differentiation). Here we demonstrate that IFN-? at relatively low concentrations (0.5?ng/mL) promoted the proliferation and migration of DPSCs, but abrogated odonto/osteogenic differentiation. Additionally, we identified that NF-?B and MAPK signaling pathways are both involved in the process of IFN-?-regulated odonto/osteogenic differentiation of DPSCs. DPSCs treated with IFN-? and supplemented with pyrrolidine dithiocarbamate (PDTC, an NF-?B inhibitor) or SB203580 (a MAPK inhibitor) showed significantly improved potential for odonto/osteogenic differentiation of DPSCs both in vivo and in vitro. These data provide important insight into the regulatory effects of IFN-? on the biological behavior of DPSCs and indicate a promising therapeutic strategy for dentin/pulp tissue engineering in future endodontic treatment.

SUBMITTER: He X 

PROVIDER: S-EPMC5241669 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature