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The rapid detection of cefotaxime-resistant Enterobacteriaceae by HPLC.

ABSTRACT:

Aim

Antibiotic resistance mediated by extended-spectrum ?-lactamases (ESBL) and AmpC ?-lactamases is widespread and increasingly common, often rendering empiric antibiotic therapy ineffective. In septicemia, delays in initiating effective antibiotic therapy are associated with worse clinical outcomes. With current phenotypic antimicrobial susceptibility testing methods, there is often a delay of 18-24 h before the susceptibility of an isolate is known.

Results

Using an HPLC assay, breakdown of the third-generation cephalosporin cefotaxime by ESBL- and AmpC- ?-lactamase-producing organisms could be detected within 90 min with 86.4% sensitivity and 100% specificity; sensitivity for ESBL detection was 100%.

Conclusion

This assay could be readily established in any clinical laboratory with an HPLC to rapidly detect ESBL-producing Enterobacteriaceae.

SUBMITTER: Robinson AM

PROVIDER: S-EPMC5242201 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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The rapid detection of cefotaxime-resistant <i>Enterobacteriaceae</i> by HPLC.

Robinson Andrew M AM Medlicott Natalie J NJ Ussher James E JE

Future science OA 20160909 4

<h4>Aim</h4>Antibiotic resistance mediated by extended-spectrum β-lactamases (ESBL) and AmpC β-lactamases is widespread and increasingly common, often rendering empiric antibiotic therapy ineffective. In septicemia, delays in initiating effective antibiotic therapy are associated with worse clinical outcomes. With current phenotypic antimicrobial susceptibility testing methods, there is often a delay of 18-24 h before the susceptibility of an isolate is known.<h4>Results</h4>Using an HPLC assay, ...[more]

PMID: 28116124

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