Dataset Information
Early retinal differentiation of human pluripotent stem cells in microwell suspension cultures.
Ontology highlight
ABSTRACT: Objective
To develop a microwell suspension platform for the adaption of attached stem cell differentiation protocols into mixed suspension culture.Results
We adapted an adherent protocol for the retinal differentiation of human induced pluripotent stem cells (hiPSCs) using a two-step protocol. Establishing the optimum embryoid body (EB) starting size and shaking speed resulted in the translation of the original adherent process into suspension culture. Embryoid bodies expanded in size as the culture progressed resulting in the expression of characteristic markers of early (Rx, Six and Otx2) and late (Crx, Nrl and Rhodopsin) retinal differentiation. The new process also eliminated the use of matrigel, an animal-derived extracellular matrix coating.Conclusions
Shaking microwells offer a fast and cost-effective method for proof-of-concept studies to establish whether pluripotent stem cell differentiation processes can be translated into mixed suspension culture.
SUBMITTER: Sharma VS
PROVIDER: S-EPMC5247545 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Publications
Early retinal differentiation of human pluripotent stem cells in microwell suspension cultures.
Biotechnology letters 20161103 2
<h4>Objective</h4>To develop a microwell suspension platform for the adaption of attached stem cell differentiation protocols into mixed suspension culture.<h4>Results</h4>We adapted an adherent protocol for the retinal differentiation of human induced pluripotent stem cells (hiPSCs) using a two-step protocol. Establishing the optimum embryoid body (EB) starting size and shaking speed resulted in the translation of the original adherent process into suspension culture. Embryoid bodies expanded i ...[more]