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MicroRNA-146a rs2910164 is associated with severe preeclampsia in Black South African women on HAART.

ABSTRACT: South African (SA) Black women have a high prevalence of preeclampsia and HIV, both conditions associated with increased inflammation. miR-146a is an inflammatory-associated miR and a common single nucleotide polymorphism (rs2910164) has been associated with several disease conditions. To date, this SNP has not been investigated in SA Black women. We therefore aimed to investigate the miR-146a G?>?C SNP in SA Blacks with preeclampsia, and further examine possible association among preeclamptic (PE) women with HIV infection on HAART.This hospital-based, case-control study included 95 normotensive and 98 PE Black SA women (aged 16-46 years old). Patients and controls were genotyped by PCR-RFLP. Using a Cytometric Bead Array assay, serum cytokine levels (including Th1- and Th2-related cytokines) were determined in 4 groups of pregnant women, viz: normotensive, HIV infected, PE?+?HIV infected, and PE women.There was no significant association between the miR-146a polymorphism and PE susceptibility in our data. However, in the subgroup analyses, the variant genotypes (GC/CC) were significantly associated with lower severe PE risk (p?=?0.0497), more especially in the presence of HIV and HAART (p?=?0.017). In the normotensive group, the variant genotypes were associated with lower IL-2 in both the total normotensive group (269?±?1.26 (36) vs 273?±?1.31 (23); p?=?0.035) and the PE HIV+ sub-group 265?±?1.54 (19) vs 271?±?1.38 (11); p?=?0.008).Our study suggests that miR-146a rs2910164 polymorphism might not be associated with PE susceptibility, cytokines or related features. However, the miR-146a GC/CC genotype might reduce susceptibility to severe PE, which might be further influenced by the presence of co-morbid HIV infection among pregnant women on HAART. This variant genotype may also be associated with reduced circulating IL-2 levels and thus reduced pro-inflammatory response in normotensive women, which may be further influenced by the presence of HIV infection and HAART.

SUBMITTER: Maharaj NR

PROVIDER: S-EPMC5248445 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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MicroRNA-146a rs2910164 is associated with severe preeclampsia in Black South African women on HAART.

Maharaj Niren Ray NR Ramkaran Prithiksha P Pillay Siddharthiya S Chuturgoon Anil Amichund AA

BMC genetics 20170119 1

<h4>Background</h4>South African (SA) Black women have a high prevalence of preeclampsia and HIV, both conditions associated with increased inflammation. miR-146a is an inflammatory-associated miR and a common single nucleotide polymorphism (rs2910164) has been associated with several disease conditions. To date, this SNP has not been investigated in SA Black women. We therefore aimed to investigate the miR-146a G > C SNP in SA Blacks with preeclampsia, and further examine possible association a ...[more]

PMID: 28103790

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Project description:BACKGROUND:Psoriasis and psoriatic arthritis (PsA) are inflammatory associated autoimmune disorders. MicroRNA (miR)-146a plays a crucial role in regulating inflammation. A single nucleotide polymorphism in the miR-146a gene (rs2910164), aberrantly alters its gene expression and linked with the pathogenesis of several disorders, including psoriasis and PsA. In South Africa, psoriasis and PsA are extremely rare in the indigenous African population and most common in both the Indian and Caucasian population. The aim of this study was to investigate whether the miR-146a rs2910164 contributes towards psoriasis and PsA development in South African Indian and Caucasian patients. METHODS:South African Indian (n?=?84) and Caucasian (n?=?32) PsA patients (total n?=?116) and healthy control subjects (Indian: n?=?62 and Caucasian: n?=?38; total n?=?100) were recruited in the study. DNA was extracted from whole blood taken from all subjects, and genotyped for the miR-146a rs2910164 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Data for laboratory parameters were obtained from pathology reports. The consulting rheumatologist collected all other clinical data. RESULTS:Unstratified data (Caucasians?+?Indians): A significant decrease in C-reactive protein (CRP) levels in PsA patients was observed (CRP monitored at inclusion vs. after 6 months of treatment) (18.95?±?2.81 mg/L vs. 9.68?±?1.32 mg/L, p?=?0.0011). The miR-146a rs2910164 variant C-allele frequency in PsA patients was significantly higher vs. healthy controls (35.78% vs. 26% respectively, p?=?0.0295, OR?=?1.59 95% CI 1.05-2.40). Stratified data (Indians): The variant C-allele frequency in Indian PsA patients was significantly higher vs. healthy Indian controls (35.71% vs. 22.58%, p?=?0.0200, OR?=?1.91 95% CI 1.13-3.22). Stratified data (Caucasians): The variant C-allele frequency distribution between Caucasian PsA patients and healthy Caucasian controls was similar. CONCLUSION:The rs2910164 variant C-allele may play a role in the progression of PsA in the South African Indian population. The main limitation in this study was the small sample size in the case-control cohorts, with a low overall statistical power (post-hoc power analysis?=?19%).

Dataset Information

MicroRNA-146a rs2910164 is associated with severe preeclampsia in Black South African women on HAART.

Publications

MicroRNA-146a rs2910164 is associated with severe preeclampsia in Black South African women on HAART.

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