Ontology highlight
ABSTRACT:
SUBMITTER: Bi Y
PROVIDER: S-EPMC4987667 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Bi Yanzhen Y Hua Zaidong Z Liu Ximei X Hua Wenjun W Ren Hongyan H Xiao Hongwei H Zhang Liping L Li Li L Wang Zhirui Z Laible Götz G Wang Yan Y Dong Faming F Zheng Xinmin X
Scientific reports 20160817
Predictable, clean genetic modification (GM) in livestock is important for reliable phenotyping and biosafety. Here we reported the generation of isozygous, functional myostatin (MSTN) knockout cloned pigs free of selectable marker gene (SMG) by CRISPR/Cas9 and Cre/LoxP. CRISPR/Cas9-mediated homologous recombination (HR) was exploited to knock out (KO) one allele of MSTN in pig primary cells. Cre recombinase was then used to excise the SMG with an efficiency of 82.7%. The SMG-free non-EGFP cells ...[more]